PRODUCTS FROM THEWELL BIOSCIENCE
3D hPSC Scale-Up with VitroGel STEM
VitroGel STEM from TheWell Bioscience is a synthetic, xeno-free hydrogel system designed to overcome the limitations of traditional 2D culture for Human Pluripotent Stem Cells (hPSCs). It enables rapid, consistent 3D expansion while preserving pluripotency.
Challenges of Traditional 2D Systems
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Inconsistent Quality: Uneven nutrient distribution leads to heterogeneous cell populations.
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Limited Scalability: Labor-intensive manual processes lack industrial capacity.
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Reproducibility Issues: High variability impacts cell consistency during scale-up.
Key Advantages of VitroGel STEM
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High Quality: Yields 3D stem cells optimized for downstream differentiation.
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Xeno-Free: 100% synthetic, biofunctional hydrogel (animal/human origin-free).
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Rapid Expansion: Faster growth rates than 2D, producing uniform spheroids.
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5-Minute Protocol: Simple mixing steps; cells can be seeded directly from liquid nitrogen.
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Easy Harvesting: Efficient recovery via centrifuge or non-enzymatic solutions.
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Versatile: Compatible with most hPSC media and standard culture vessels.
Integrated Product System
- VitroGel STEM: Xeno-free hydrogel for rapid 3D expansion.
- RocketCell Media: All-in-one growth kit optimized for 3D iPSCs and hESCs.
- VitroPrime Vessels: Plates featuring “sample-locking” and media-exchange channels.
Seamless Transition Workflow
The system supports a gradual transition from 2D to 3D to help researchers understand changing cell behaviors:
- 2D Hydrogel Coating: A simple bridge ensuring growth and attachment.
- 3D Cell Culture: Time-saving protocol to maintain pluripotent properties.
- Static Suspension Culture: The ultimate scale-up method for efficient, cost-effective spheroid formation directly from cryovials.
*All products listed on this site and sold through LAB-A-PORTER Limited are for research use only (RUO). Not for human use. Not for therapeutic or diagnostic use.