VITROGEL® HYDROGEL

User-friendly and robust hydrogel for 3D cell culture research

VitroGels are easy-to-use, xeno-free tunable hydrogels that closely mimic the natural extracellular matrix (ECM) environment. The hydrogel system is ready-to-use at room temperature, has a neutral pH, transparent, permeable and compatible with different imaging systems. The tunability of the biophysical and biological properties of the hydrogel can create an optimized environment for cell growth. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. The hydrogel strength can be adjusted with the VitroGel Dilution Solution. Cells cultured in this system can be easily harvested out with the VitroGel Cell Recovery Solution. The hydrogel is injectable for in vivo studies.

  • Choose hydrogels with functional ligands such as RGD, collagen, laminin and MMP.
  • “Mix & Match” Unique to the VitroGel system is the ability to customized multi-functional hydrogel by blending different types of VitroGel together.

VITROGEL IS A XENO-FREE TUNABLE HYDROGEL SYSTEM

Closely mimicking the natural extracellular matrix (ECM) environment, this distinct system brings many advantages to bridge
in vitro and in vivo studies through 3D cell culture and beyond.

Easy-to-use

Mix with cells at room temperature, add medium and incubate.  VItroGel is neutral in pH and no cross-linking agent required .

Xeno-free

100% animal origin-free hydrogel.

Tunable hydrogel

Adjust the hydrogel stiffness from a wide range for different applications.

Room temp stable

Get rid of the ice bucket.  VitroGel is room temperature stable and ready-to-use.

Transparent

The hydrogel is transparent and compatible with different imaging systems for cell observation.

Reproducible

Our manufacturing process and quality control ensures batch to batch consistency.

Easy cell harvesting 

20 min protocol.  Use our enzyme-free cell recovery solution to harvest cells fast while maintaining high cell viability.

Biocompatible

VitroGel supports various cell activities and safe for in vivo applications

Biofunctional modifiable

Biofunctional ligands link to hydrogel matrix through covalent bonds to induce cell-matrix interactions

Mix & Match

Blend different types of VitroGel to to create a customized multi-functional hydrogel.

Injectable

After soft gel formation, the hydrogel is injectable for cell therapy and drug delivery applications.

Automation friendly

VitroGel is temperature stable and easy for pipetting.  It is ideal for automation and high-content screening.

3D CELL CULTURE PROCESS IN 20 MIN

VitroGel comes ready to use.  There is no cross-linking agent required.  Work confidently at room temperature.  No need for an ice bucket or worry about thawing issues. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. The hydrogel strength can be adjusted with the VitroGel Dilution Solution. The xeno-free system gives scientists the control to add defined growth factors to the cell suspension. Enjoy the benefits of the setup and processing time.  With VitroGel, the 3D cell culture process can be accomplished in 20 minutes.

EASY CELL RECOVERY FROM HYDROGEL SYSTEM

As easy as it is to perform 3D or 2D cell culture with VitroGel, it is even easier to harvest cells out from the hydrogel system with the VitroGel® Cell Recovery Solution. This enzyme-free solution has a neutral pH and works at 37°C operating temperature. With a simple rocking and incubation process, cells cultured in 3D or 2D coating VitroGel can be harvested in 10-20 minutes while maintaining high cell viability.

VitroGel Cell Recovery Solution (Enzyme-free)

3D CELL CULTURE YOUR WAY

over 100 possible combination

With VitroGel system, all different functional modifications are offered in the same xeno-free tunable hydrogel 3D culture system. The system allows scientists to investigate different combinations of VitroGel® products to find the right conditions for their experiments. For example, scientists can combine different functional adhesive ligands, such VitroGel® COL with VitroGel® RGD, VitroGel® IKVAV, VitroGel® YIGSR, or VitroGel® LDP1/2/3, to form a heterogeneous, and realistic, microenvironment. Also, the MMP enzyme sensitive hydrogel, VitroGel MMP, can be used to study the degradation of the local ECM by metastatic cells for invasion and dissemination into new tissue. This key feature permits examination of cellular responses in a more physiologically relevant context, while still giving experimenters full control. Due to the diversity of VitroGel® products, a wide variety of cell types from different tissues can be tested.

Multiple studies have been made use of biofunctional hydrogel in different tissue and cell types. In nature, the microenvironment is complicated. Each single cell type can respond to different functional ligands such as RGD, Collagen, Laminin, and MMP. VitroGel hydrogel system is a powerful tool for scientists to study many cellular processes and behaviors in response to different extracellular matrix functional ligands. The table below provides a quick guideline to help customers to identify the functional hydrogel that can use for their cell types. Keep in mind there are many more possibilities that have not been explored yet. The “mix and match” function of VitroGel gives the flexibility for all possibilities.

AN INJECTABLE HYDROGEL FOR MULTIPLE APPLICATIONS

VitroGel hydrogel system has a unique shear-thinning and rapid recovery rheological property. Under the mechanical shearing force such as injection through a syringe, the hydrogel performs a gel-sol transition and becomes free-flowing status. However, once the shearing force ceased, the mechanical strength of the hydrogel can rapidly recover with a sol-gel transition and become a hydrogel status again. 

With this injectable property, VitroGel can be used for in vivo cells/drug delivery with great retention, bioprinting, droplet formation, and lab automation.

TUNABLE HYDROGEL STRENGTH

VitroGel® Dilution Solution is a ready-to-use solution use for mixing with the VitroGel system to adjust the hydrogel concentration for different hydrogel strength.

The solution is room temperature stable and neutral in pH, which maintains a good physiological condition for hydrogel formation. The VitroGel Dilution Solution can accelerate the hydrogel formation in combination with cell medium. The Dilution Solution can be used to prepare VitroGel thin gel coating plate or injectable hydrogel.

PERFORMANCE COMPARISON TO OTHER 3D CELL CULTURE METHODS

VitroGel comes in high concentration.  Get more uses from one vial.  One 10 mL vial can be used for 7 plates of 24-well plate if using dilution ratio at 1:3 which is the typical ratio for most cell types.  Overall, cost per well is more than 50% lower compared to other basement membrane matrix.

50% lower cost overall

See how VitroGel features compare to other 3D cell culture methods above.

VitroGel gives an outstanding balance of biological functions and operating feasibility. The system can closely mimic the natural extracellular matrix and allow the high-throughput performance to build robust 3D cell models.

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