User-friendly and robust hydrogel for 3D cell culture research

3D Cell Culture


Closely mimicking the natural extracellular matrix (ECM) environment, our state-of-the-art hydrogel system gives an outstanding balance of biological functions and operating ease to establish a robust 3D cell culture platform and injectable delivery system for drug discovery, tissue engineering, cell therapy, and personalized medicine.

A xeno-free system is key for 3D cell culture in clinical applications. Our cutting-edge hydrogel enables high-throughput performance to establish 3D organotypic models from both cell lines and patient-derived cells. Using our hydrogel system as an injectable delivery system, scientists can achieve better cell retention and higher cell viability for cell therapy.  The groundbreaking technology allows cell recovery from the 3D hydrogel matrix easier than ever before.

VitroGel® Hydrogel Matrix


A user-friendly functional hydrogel offering an excellent balance of simplicity and versatility.

VitroGel® Hydrogel Matrix is a ready-to-use, xeno-free functional hydrogel for 3D cell culture research. VitroGel Hydrogel Matrix is an optimized formulation of multi-functional ligands and concentration to support a wide range of cell types for different applications. The hydrogel is ready to mix with cell suspension directly. There is no additional adjustment needed.  For many users, this is the preferred hydrogel for 3D cell culture and 2D coating.

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VitroGel® High Concentration Hydrogels

Building blocks for unlimited creativity and maximum flexibility

VitroGel High Concentration Hydrogels offer a set of building blocks to create a functional micro-environment that closely mimics the natural extracellular matrix (ECM) for cells to feel like at home. The high concentration formulation allows the maximum flexibility to manipulate the mechanical strength of hydrogel from 10 to 4000 Pa. The hydrogels come with several types of functional ligand modifications such as RGD (VitroGel RGD), Collagen (VitroGel COL), Laminin (VitroGel IKVAV & VitroGel YIGSR) and MMP (VitroGel MMP). The system allows scientists to investigate the cell behaviors in response to an individual functional ligand or create multi-functional hydrogel combinations by blending different versions of VitroGel.

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VitroGel Features

Closely mimicking the natural extracellular matrix (ECM) environment, this distinct system brings many advantages to bridge
in vitro and in vivo studies through 3D cell culture and beyond.


Mix with cells at room temperature, add medium and incubate.  VItroGel is neutral in pH and no cross-linking agent required .


100% animal origin-free hydrogel.

Tunable hydrogel

Adjust the hydrogel stiffness from a wide range for different applications.

Room temp stable

Get rid of the ice bucket.  VitroGel is room temperature stable and ready-to-use.


The hydrogel is transparent and compatible with different imaging systems for cell observation.


Our manufacturing process and quality control ensures batch to batch consistency.

Easy cell harvesting 

20 min protocol.  Use our enzyme-free cell recovery solution to harvest cells fast while maintaining high cell viability.


VitroGel supports various cell activities and safe for in vivo applications

Biofunctional modifiable

Biofunctional ligands link to hydrogel matrix through covalent bonds to induce cell-matrix interactions

Mix & Match

Blend different types of VitroGel to to create a customized multi-functional hydrogel.


After soft gel formation, the hydrogel is injectable for cell therapy and drug delivery applications.

Automation friendly

VitroGel is temperature stable and easy for pipetting.  It is ideal for automation and high-content screening.


As easy as it is to perform 3D or 2D cell culture with VitroGel, it is even easier to harvest cells out from the hydrogel system with the VitroGel® Cell Recovery Solution. This enzyme-free solution has a neutral pH and works at 37°C operating temperature. With a simple rocking and incubation process, cells cultured in 3D or 2D coating VitroGel can be harvested in 10-20 minutes while maintaining high cell viability.

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VitroGel Cell Recovery Solution (Enzyme-free)


VitroGel hydrogel system has a unique shear-thinning and rapid recovery rheological property. Under the mechanical shearing force such as injection through a syringe, the hydrogel performs a gel-sol transition and becomes free-flowing status. However, once the shearing force ceased, the mechanical strength of the hydrogel can rapidly recover with a sol-gel transition and become a hydrogel status again. 

With this injectable property, VitroGel can be used for in vivo cells/drug delivery with great retention, bioprinting, droplet formation, and lab automation.


VitroGel® Dilution Solution is a ready-to-use solution use for mixing with the VitroGel system to adjust the hydrogel concentration for different hydrogel strength.

The solution is room temperature stable and neutral in pH, which maintains a good physiological condition for hydrogel formation. The VitroGel Dilution Solution can accelerate the hydrogel formation in combination with cell medium. The Dilution Solution can be used to prepare VitroGel thin gel coating plate or injectable hydrogel.


VitroGel comes in high concentration.  Get more uses from one vial.  One 10 mL vial can be used for 7 plates of 24-well plate if using dilution ratio at 1:3 which is the typical ratio for most cell types.  Overall, cost per well is more than 50% lower compared to other basement membrane matrix.

50% lower cost overall

See how VitroGel features compare to other 3D cell culture methods above.

VitroGel gives an outstanding balance of biological functions and operating feasibility. The system can closely mimic the natural extracellular matrix and allow the high-throughput performance to build robust 3D cell models.

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