Download application note: Differentiation of induced pluripotent stem cells (iPSCs) into neuroectoderm
The differentiation of feeder-free human induced pluripotent stem cells (iPSCs) into neuroectodermal cells is a complex process that uses growth factors such as noggin and FGF-2. Additionally, factors such as
activin receptor-like kinase receptor blocker SB431542 are required to improve the efficiency of differentiation and stabilize the neuroectodermal fate. This combination allows researchers to reliably generate functional neuroectodermal cells for various biomedical applications. In this application note, we outline the method for differentiating iPSCs into neuroectoderm cells using media containing members of our recombinant FGF-2 family, such Qkine FGF2-G3 (Qk053), together with noggin (Qk034). Neuroectoderm marker expression is used to assess successful differentiation via immunocytochemistry.
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ABOUT QKINE
A UK Manufacturer Provides Cost-Effective Highly Bioactive Growth Factors For Stem Cell and Organoid Research
Qkine manufactures high-purity, animal-free growth factors, cytokines and other complex proteins for stem cell and organoid culture. An ISO 9001: 2015 certified company based in Cambridge, UK, Qkine combines proprietary production processes with protein engineering technology to tackle fundamental biological and scale-up challenges.
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- Highly bioactive
- Animal-free to eliminate the risks of contaminating growth factors, viruses, and prions
- Manufactured in UK
- Clear QC data provided on every lot from our ISO 9001:2015 accredited facility
- Excellent lot-to-lot consistency to help increase the reproducibility of experiments
- High purity
- Long-term stability
- Multiple pack sizes to suit your research