Organoid and 3D Cell Culture Recipe

by | Oct 30, 2019 | Organoids

Retinal Organoid. This image (Credit: Anand Swaroop, Ph.D., NEI/NIH) is used under Public Domain Mark 1.0: https://creativecommons.org/publicdomain/mark/1.0/

INTRODUCTION

Organoid

 

Organoids are three-dimensional (3D) miniature structures cultured in vitro produced from either human pluripotent stem cells (hPSCs) or adult stem cells (AdSCs) derived from healthy individuals or patients that recapitulate the cellular heterogeneity, structure, and functions of human organs [1]. With the arrival of human 3D organoid systems, it is now possible to grant specific examination of stem cell morphogens, maintenance, and differentiation that are look-alike primary tissues, reinforcing the potential to research both human physiology and developmental stage[1]. At the same time, they are very much alike to their original organs and carry human genetic information, organoids derived from patients hold great promise for biomedical research and preclinical drug testing and are currently used for personalized, regenerative medicine, gene repair, and transplantation therapy[1]. In recent decades, researchers have successfully generated various types of organoids mimicking in vivo organs[1].

Emerging organoid technologies have brought opportunities for biomedical research[1]. The broader applications of organoids are in the beginning stage of exploration[1]. Extensive research will enable 3D organoid systems to replenish existing model systems, which could enhance basic and clinical studies in the future[1].

Reference:

[1] Tang, Xiao-Yan et al. “Human organoids in basic research and clinical applications.” Signal transduction and targeted therapy vol. 7,1 168. 24 May. 2022, doi:10.1038/s41392-022-01024-9

SELECTED ORGANOID REAGENTS AND TOOLS BY LAB-A-PORTER

Our Popular Organoid Reagents By Research Interests

Blood Vessel Organoid

Growth factors

  • BMP-4

  • FGF-basic

  • VEGF-A

 

    Xeno-free 3D hydrogel and medium

    • Xeno-free 3D hydrogel for organoid
    • Organoid Recovery Solution
    Brain Organoid

    Growth factors

    • BDNF

    • DKK-1

    • EGF

    • FGF-basic

    • GDNF

    • Noggin

    Small molecules

    • Dorsomorphin

    • GDC-0068

    • MK-2206

    • Y-27632

    Xeno-free 3D hydrogel and medium

    • Xeno-free 3D hydrogel for organoid
    • Organoid Recovery Solution
    Endometrium Organoid

    Growth factors

    • EGF

    • FGF-10

    • HGF

    • Noggin

    • R-Spondin 1

    Xeno-free 3D hydrogel and medium

    • Xeno-free 3D hydrogel for organoid
    • Organoid Recovery Solution
    Esophagus Organoid

    Growth factors

    • Activin A
    • BMP-4
    • EGF
    • FGF-basic
    • FGF-10
    • KGF
    • Noggin
    • Wnt-3a

    Xeno-free 3D hydrogel and medium

    • Xeno-free 3D hydrogel for organoid
    • Organoid Recovery Solution
    Fallopian Tube Organoid

    Growth factors

    • EGF

    • FGF-10

    • Noggin

    • R-Spondin 1

    • Wnt-3a

     

    Xeno-free 3D hydrogel and medium

    • Xeno-free 3D hydrogel for organoid
    • Organoid Recovery Solution
    Gallbladder Organoid

    Growth factors

    • EGF
    • FGF-10
    • HGF
    • Noggin
    • R-Spondin 1

      Xeno-free 3D hydrogel and medium

      • Xeno-free 3D hydrogel for organoid
      • Organoid Recovery Solution
      Heart Organoid

      Growth factors

      • FGF-basic

      • TGF-beta 1

        Xeno-free 3D hydrogel and medium

        • Xeno-free 3D hydrogel for organoid
        • Organoid Recovery Solution
        Inner Ear Organoid

        Growth factors

        • BMP-4

        • FGF-basic

        Small molecules

        • A 83-01

        • LDN-193189

        • SB 431542

        Xeno-free 3D hydrogel and medium

        • Xeno-free 3D hydrogel for organoid
        • Organoid Recovery Solution
        Intestinal Organoid

        Growth factors

        • Activin A

        • EGF

        • FGF-basic

        • FGF-4

        • IGF-1

        • Noggin

        • R-Spondin 1

        • Wnt-3a

        Small molecules

        • A 83-01

        • Nicotinamide

        • SB 202190

        • Y-27632

        Xeno-free 3D hydrogel and medium

        • Xeno-free 3D hydrogel for organoid
        • Organoid Recovery Solution
        Kidney Organoid

        Growth factors

        • Activin A

        • BMP-2

        • BMP-4

        • BMP-7

        • FGF-basic

        • FGF-9

        Small molecules

        • CHIR 99021

        • Retinoic Acid

        • Y-27632

         

          Xeno-free 3D hydrogel and medium

          • Xeno-free 3D hydrogel for organoid
          • Organoid Recovery Solution
          Liver Organoid

          Growth factors

          • Activin A

          • BMP-4

          • BMP-7

          • EGF

          • FGF-basic,

          • FGF-7

          • FGF-10

          • FGF-19

          • HGF

          • KGF

          • Noggin

          • OSM

          • R-Spondin 1

          • Wnt-3a

          • TGF-alpha

          Small molecules

          • A 83-01
          • DAPT
          • Forskolin
          • Gastrin
          • Nicotinamide
          • Prostaglandin E2
          • Y-27632

          Xeno-free 3D hydrogel and medium

          • Xeno-free 3D hydrogel for organoid
          • Organoid Recovery Solution
          Lung Organoid

          Growth factors

          • Activin A

          • FGF-basic

          • FGF-4

          • Noggin

          • FGF-10

          Small molecules

          • CHIR 99021
          • SB 431542

           

            Xeno-free 3D hydrogel and medium

            • Xeno-free 3D hydrogel for organoid
            • Organoid Recovery Solution
            Mammary Organoid

            Growth factors

            • IGF-1
            • Shh
            • Wnt-3a

            Small molecules

            • CHIR 99021
            • Y-27632

            Xeno-free 3D hydrogel and medium

            • Xeno-free 3D hydrogel for organoid
            • Organoid Recovery Solution
            Oral Mucosa Organoid

            Growth factors

            • EGF
            • FGF-basic
            • FGF-10
            • Noggin
            • R-Spondin 1

            Xeno-free 3D hydrogel and medium

            • Xeno-free 3D hydrogel for organoid
            • Organoid Recovery Solution
            Ovarian Surface Epithelium Organoid

            Growth factors

            • EGF

            • NRG-1

            • Noggin

            • R-Spondin 1

            • Wnt3a

             

              Xeno-free 3D hydrogel and medium

              • Xeno-free 3D hydrogel for organoid
              • Organoid Recovery Solution
              Pancreatic Organoid

              Growth factors

              • Activin A

              • BMP-4

              • EGF

              • FGF-4

              • FGF-10

              • Noggin

              • R-Spondin 1

              • Wnt-3a

              Small molecules

              • A 83-01
              • Gastrin,
              • Nicotinamide

              Xeno-free 3D hydrogel and medium

              • Xeno-free 3D hydrogel for organoid
              • Organoid Recovery Solution
              Prostate Organoid

              Growth factors

              • Activin A

              • EGF

              • FGF-basic

              • FGF-10

              • Noggin

              • R-Spondin 1

              Small molecules

              • A 83-01

              • SB 202190

              • Y-27632

              • Prostaglandin E2

              • Nicotinamide

                Xeno-free 3D hydrogel and medium

                • Xeno-free 3D hydrogel for organoid
                • Organoid Recovery Solution
                Retina Organoid

                Growth factors

                • IGF-1
                • Shh
                • Wnt-3a

                Small molecules

                • CHIR 99021
                • Y-27632

                Xeno-free 3D hydrogel and medium

                • Xeno-free 3D hydrogel for organoid
                • Organoid Recovery Solution
                Skin Organoid

                Growth factors

                • BMP-4

                • FGF-basic

                Xeno-free 3D hydrogel and medium

                • Xeno-free 3D hydrogel for organoid
                • Organoid Recovery Solution
                Stomach Organoid

                Growth factors

                • Activin A

                • EGF

                • FGF-10

                • Noggin

                • R-Spondin 1

                • Wnt-3a

                 

                Small molecules

                • A 8301

                • Gastrin

                • Nicotinamide

                • SB 202190

                • Y-27632

                 

                Xeno-free 3D hydrogel and medium

                • Xeno-free 3D hydrogel for organoid
                • Organoid Recovery Solution
                Urothelium Organoid

                Growth factors

                • FGF-basic
                • FGF-7
                • FGF-10

                 

                  Xeno-free 3D hydrogel and medium

                  • Xeno-free 3D hydrogel for organoid
                  • Organoid Recovery Solution

                  *All products listed on this site and sold through LAB-A-PORTER Limited are for research use only (RUO). Not for human use. Not for therapeutic or diagnostic use.

                  CONTACT US TO LEARN MORE.

                  Contact us
                  Share This