Retinal Organoid. This image (Credit: Anand Swaroop, Ph.D., NEI/NIH) is used under Public Domain Mark 1.0: https://creativecommons.org/publicdomain/mark/1.0/
Organoid models include three-dimensional (3D) cell culture systems that closely resemble in vivo organ or tissue. These 3D systems reproduce the complex spatial morphology of a differentiated tissue to allow biologically relevant cell-cell and cell-matrix interactions, ideally, sharing similar physiological responses with in vivo differentiated tissues.
The major focus of future organoid studies, beside the study of developmental processes, will most likely be in drug testing and disease modeling, such as developmental disorders, genetic conditions, cancer and degenerative disorders.
The hope is that organoids are another step in the long way towards in vitro construction of tissues and organs for transplantation in patients.
Selected compositions for organoid cultures by LAB-A-PORTER
KIDNEY ORGANOIDS
Proteins & Growth Factors
Small Molecules
3D Growth Matrix
References
Xia Y. et al. (2014) Nature Protocols. 9:2693.
Xia Y. et al. (2013) Nature Cell Biology. 15:1507.
Takasato M. et al. (2014) Nat. Cell Biol. 16:118
INNER EAR ORGANOIDS
Proteins & Growth Factors
Small Molecules
3D Growth Matrix
References
Koehler K.R. and E. Hashino (2014) Nat. Protocols. 9:1229.
RETINA ORGANOIDS
Proteins & Growth Factors
Small Molecules
3D Growth Matrix
References
Nakano T. et al. (2012) Cell stem cell. 10: 771
STOMACH ORGANOIDS
Proteins & Growth Factors
Small Molecules
3D Growth Matrix
References
Mahe M. et al. (2013) Curr. Protoc. Mouse Biol. 3:217
Sato T. and H. Clevers (2015) Cell. 161:1700
Bartfeld S. et al. (2015) Gastroenterology. 148:126
LIVER ORGANOID
Proteins & Growth Factors
Small Molecules
3D Growth Matrix
References
Mahe M. et al. (2013) Curr. Protoc. Mouse Biol. 3:217
Sato T. and H. Clevers (2015) Cell. 161:1700
Bartfeld S. et al. (2015) Gastroenterology. 148:126
PROSTATE ORGANOID
Proteins & Growth Factors
Small Molecules
- A 83-01
- SB 202190
- Y 27632 Dihydrochloride
- Prostaglandin E2
- Nicotinamide
- Testosterone
3D Growth Matrix
References
Karthaus W.R. et al. (2014) Cell. 159:163.
Drost J. et al (2016) Nat. Protocols. 11:347
BRAIN ORGANOIDS
Proteins & Growth Factors
Small Molecules
3D Growth Matrix
References
Lancaster M.A. and J. A. Knoblich (2014) Nat. Protocols.
9:2329
Mariani J. et al. (2015) Cell. 162:375
Li Y. et al. (2017) Cell Stem Cell 20:1
IMMUNO GERMINAL CENTER-LIKE ORGANOIDS
Proteins & Growth Factors
3D Growth Matrix
References
Purwada A. and Singh A. (2017) Nat. Protocols. 12:168
MAMMARY ORGANOIDS
Proteins & Growth Factors
Small Molecules
3D Growth Matrix
References
Jardé T.et al. (2016) Nat. Commun. 7: 13207
Jamieson PR. et al. (2016) Develompent. :dev-145045
SMALL INTESTINAL AND COLONIC ORGANOIDS
Proteins & Growth Factors
Small Molecules
3D Growth Matrix
References
Mahe M. et al. (2013) Curr. Protoc. Mouse Biol. 3:217
Sato T. et al. (2011) Gastroenterology. 141:1762
Sato T. and H. Clevers (2015) Cell. 161:1700
Sato T. and H. Clevers (2009) Nature. 459:262
Jung P. et al. (2011) Nature Medicine. 17:1225
PANCREATIC ORGANOID
Proteins & Growth Factors
Small Molecules
3D Growth Matrix
References
Sato T. and H. Clevers (2015) Cell. 161:1700
Boj S.F. et al. (2015) Cell. 160:324.
Broutier L. et al. (2016) Nature Protocols. 11:1724
LUNG ORGANOID
Proteins & Growth Factors
Small Molecules
3D Growth Matrix
References
Dye B.R. et al. (2015) eLIFE. 4:e05098