MS VALIDATED ANTIBODIES
Extensively validated, high-quality antibodies are key to successful experiments. Optimal antibodies are particularly critical for multiplex fluorescence immunohistochemistry where even one cross-reactive antibody can spoil an entire experiment. Based in Hamburg, Germany, MS Validated Antibodies is devoted to delivering optimal monoclonal antibodies for immunohistochemistry and to providing the users with unique product documentation enabling them to better know their antibodies.
ABOUT THE COMPANY
Address the pain points in immunohistochemistry
Many antibody vendors provide very little information and images on how an antibody stains. References are often limited to a positive and a negative control on their website. When pathologists and researchers use these antibodies, they don’t know about the complete staining behaviour of an antibody because often they do not have time or access to enough different tissues to conduct an in-house validation of the antibody. This can cause severe problems because researchers cannot know if they can trust the antibody and the data they have generated in their studies.
Large-scale tissue-validated antibodies designed for immunochemistry
MS Validated Antibodies is devoted to delivering optimal monoclonal antibodies for immunohistochemistry and to provide the users with a unique product documentation enabling them to know as much as possible on their antibodies. Key features of the MSVA package include:
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- Highly stringent criteria for product selection: Only 150 out of the first 5500 evaluated antibodies were selected for the MSVA portfolio.
- Unique product documentation including images from more than 50 different normal tissues and more than 20 tumor images per antibody.
- Protocol suggestions for manual staining and autostainers (Dako, Leica, Ventana).
- Published data on the performance characteristics of our antibodies are compiled on the MSVA homepage (if available).
- Extensive educational content is provided for every antibody including links to other relevant sites.
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Antibodies for multiplex fluorescence immunochemistry
Extensively validated, high-quality antibodies are key to successful experiments. Optimal antibodies are particularly critical for multiplex fluorescence immunohistochemistry where even one cross-reactive antibody can spoil an entire experiment.
PRODUCT FEATURES
1. Hands-on validation approach. No cross-reactivity.
Antibodies intended for use on formalin-fixed tissue must be specifically validated on formalin-fixed tissue. According to the International Working Group for Antibody Validation (IWGAV), there are only two ways to document the specificity of antibodies for immunohistochemistry on this type of tissue. These are: Orthogonal strategy & Independent antibody strategy.
To ensure the best possible validation and to avoid the problem of non-specific antibodies, MS Validated Antibodies combine both approaches. The resulting abundance of information on the antibodies is available on our website.
2. Provide up to 100 images for each antibodies
MS Validated Antibodies is dedicated to providing users with product documentation that allows them to better understand their antibodies, which is key to the successful use of an antibody in IHC. They provide up to 100 images of each antibody in a normal tissue gallery and a cancer tissue gallery.
3. Large-scale studies
It is often not known to researchers and pathologists where certain antibodies should stain and literature data on certain proteins show inconsistent results. In order to get a true picture of the staining behavior of an antibody MS Validated Antibodies supports IHC large-scale studies on thousands of different tumor samples.
4. Multiplex fluorescence immunohistochemistry
Multiplex fluorescence immunohistochemistry (mfIHC) allows multiple antibodies to be applied to a tissue slide. Sophisticated analysis frameworks use artificial intelligence to identify specific cell types defined by a combination of positive and negative antibody staining and the spatial relationship between those cell types.
Due to the high number of different “colors” on each stained slide and the impaired differentiation of morphological cell types in fluorescence imaging, visual quality control of individual antibodies becomes impossible. Non-specific or cross-reactive antibody staining of one or more antibodies used in a multicolor panel can seriously distort the data and conclusions from the experiments.
Hence the quality of the composite of antibodies used in a mfIHC panel is crucial.
