iNtRON BIOTECHNOLOGY

Based in Korea, with an international office in Boston, US, iNtRON Biotechnology is a KOSDAQ-listed manufacturer providing diagnostic tools based on PCR technologies and various research-use-only (RUO) tools on genomic research, proteomic research and cell culture. 

LILIF DIAGNOSTIC

(A trademark of iNtRON Biotechnology)

LiliF® is a trademark of iNtRON Biotechnology which provides diagnostic kits, research-use-only (RUO) kits and reagents. iNtRON’s production system is certified by ISO9001:14000, ISO13485:GMP System on their product quality and manufacturing consistency.

PRODUCT PORTFOLIO

Based in Korea, with an international office in Boston, US, iNtRON Biotechnology provides various research-use-only tools on genomic research, proteomic research and cell culture, specializing products in nucleic acid extraction and amplifications, protein extraction,  as well as mycoplasma detection and prevention. Key products include:

 

  • Genomic DNA Extraction Kits
  • Plasmid DNA Purification Kits
  • RNA Extraction Kits
  • Protein Extraction Solution and Western Blot Detection System
  • Tools for PCR, RT-PCR and Real-time PCR
  • Mycoplasma PCR Detection Kits and Mycoplasma Prevention Spray

NEW PRODUCTNew

RealMOD™ Probe R² 2X qRT-PCR mix (with UDG)

Real-time RT-PCR (qRT-PCR) is the preferred method for RNA quantification because of its high sensitivity, reproducibility and wide dynamic range. Recently, the importance of accuracy has emerged in molecular diagnosis by using Real-time RT-PCR.

RealMOD™ Probe R2 2X qRT-PCR mix (with UDG) is a 2X concentration premix type reagent specially designed for Real-time RT-PCR by using TaqMan probe. This kit contains all necessary reagents (DNA Polymerase, UDG, reverse transcriptase, ultrapure dNTPs, dUTP, MgCl2 etc.) for Real-time RT-PCR reaction except for primers, probe and template RNA.

UDG-system reagents, dUTP and thermolabile UDG, are included in the mixture to prevent the reamplification of cross/carry over PCR products between reactions.

  • dUTP in the mixure ensures that any amplified cDNA will contain uracil.
  • UDG removes uracil residues from single- or double-stranded cDNA, preventing uracil containing cDNA from serving as template in future PCRs.
  • Anti Taq antibody based on Hot-start DNA polymerase prevents extension of non specifically annealed primers and primer-dimer formation at low temperatures during qRT-PCR setup.

*All products listed on this site and sold through LAB-A-PORTER Limited are for research use only (RUO). Not for human use. Not for therapeutic or diagnostic use.

FEATURED PRODUCTS

DNA & RNA Extraction Kits

G-spin™ Total DNA Extraction Mini Kit

G-spin™ Total DNA Extraction Mini Kit provides fast and easy methods for purification of total DNA from cultured animal cell, animal tissue, rodent tail, fixed tissue, animal hair, gram negative bacteria, and blood sample for reliable PCR and Southern blotting.

DNA-spin™ Plasmid DNA Purification Kit

DNA-spin™ Plasmid DNA Purification Kit provide a fast, efficient means of preparing high purity plasmid DNA without specialized devices or equipment.

This kit contains a spin-type column filled with silica bead membrane and reagents optimizing alkali lysis for easy purification of plasmid DNA from bacteria.

DNA-midi™ GT Plasmid DNA Purification Kit

DNA-Midi™ GT Plasmid DNA Purification Kit is designed for efficient purification of high quality plasmid DNA from bacterial cells.

This kit provides alkaline lysis reagents and columns packed with anion-exchanger resins.

The entire midi prep process can be completed within 120 minutes through gravity-flow procedure.

MEGAquick-spin™ Plus Total Fragment DNA Purification Kit

The MEGAquick-spinTM Plus Total Fragment DNA Purification Kit is designed to extract and purify DNA fragments of 65 bp ~ 10 kb from normal or low-melt agarose gels in either Tris acetate (TAE) or Tris borate (TBE), or to purify PCR products directly from a PCR amplification and DNA cleanup from other enzymatic reactions.

easy-spin™ Total RNA Extraction Kit

easy‐spin™ (DNA free) Total RNA Extraction Kit combines the advantages of solution type and column type DNA extraction products in the market, removing the inconveniency of alcohol PPT process in solution type products and enabling the extraction of total RNA within 30 minutes without genomic DNA.

The most remarkable characteristics of the easy-spin™ Kit is: (1) there is no genomic DNA contamination,(2) there is no alcohol PPT process, and (3) as a result RNA extraction time is less then 30 minutes.

Mycoplasma PCR Detection Kits & Mycoplasma Prevention Spray

Mycoplasma is a genus of bacteria which lack a cell wall. Without a cell wall, they are unaffected by many common antibiotics or other beta-lactam antibiotics that target cell wall synthesis. They can be parasitic or saprotrophic.

Mycoplasma are common and serious contaminants of cell cultures. It has been shown that 30% to 87% of cell cultures are infected with mycoplasma. Many mycoplasma contaminations, particularly in continuous cell lines, grow slowly and do not destroy host cells but are still able to affect various parameters, leading to unreliable or false results. These effects include changes in metabolism, growth, viability, DNA, RNA, and protein synthesis, and morphology. Testing for mycoplasma is an essential quality control tool to assure accurate research and reliable biotechnological products

e-Myco™ Mycoplasma PCR Detection Kit (ver.2.0)

The e-Myco™ (ver.2.0) product is a set of primers designed to detect the presence of mycoplasma that might contaminate biological materials such as cultured cells. Five common cell culture-infecting species of mycoplasma and also other various species of mycoplasma can be detected.

Each tube of the e-Myco™ Mycoplasma PCR Detection Kit (ver.2.0) contains all the components for PCR except for template:

  • i-StarTaqTM DNA Polymerase
  • dNTPs, 10x Buffer, primers
  • 8-MOP
  • internal control for mycoplasma partial gene amplifications

e-Myco™ plus Mycoplasma PCR Detection Kit

Specific primers set of e-Myco™ plus Mycoplasma PCR Detection Kit were designed from DNA sequences that are coding for highly conserved 16S rRNA with considering above point. e-Myco™ plus Mycoplasma PCR Detection Kit can be used in the detection of a more broad range of Mycoplasma species, compared with any other commercially available PCR-based Mycoplasma detection kit, without interfering with animal or bacterial DNA.

Not only five common cell cultureinfecting species of mycoplasma but also other various species of mycoplasma over 8 genus 209 species (list of species refer to attachment) can be detected.

e-Myco™ VALiD Mycoplasma PCR Detection Kit

e-Myco™ VALiD Mycoplasma PCR Detection Kit is composed a set of primers those are specific for the highly conserved mycoplasma 16S-rRNA coding region including M. pneumoniae, M. agninini, M. hyorhinis, M. fermentans, M. orale and A. laidlawii. The kit is design to detect the presence of mycoplasma that might contaminate biological materials such ascultured cells.

Also, the kit can be performed in 3 hours, can detect sensitively until 10 CFU/ml and includes internal control for verifying a PCR run as well as positive control DNA.

Each tube of the e-Myco™ VALiD Mycoplasma PCR Detection Kit provides all-in-one system (FastMix technology), which means all components for PCR is already pre-aliquoted in each PCR tube.

MycoClean™ Mycoplasma Prevention Spray

MycoClean™ Mycoplasma Prevention Spray is an effective solution for reliable disinfection of laboratory surfaces and apparatus including clean benches, incubators, work benches, cell storage boxes and liquid nitrogen containers.

MycoClean™ shows an excellent effectiveness against mycoplasma. Contamination-fee and clean surfaces can be achieved in single application.

Ready to use PCR PreMix Kits – Taq DNA Polymerase, dNTP mixture and reaction buffer in one tube

Taq DNA Polymerase is the most common PCR enzyme for amplifying up to 10kb lDNA templates and up to 3kb genomic templates. However, it is not able to amplify even longer DNA fragments. To overcome this constraint, variable PCR systems have been developed by various company, which are containing Taq DNA Polymerase and thermostable DNA Polymerase with proofreading activity.

iNtRON’s Maxime PCR PreMix Kit has not only various kinds of PreMix Kit according to experience purpose, but also a 2X Master mix solution.

Maxime™ PCR PreMix (i-Taq)

Maxime PCR PreMix Kit (i-Taq) is the product what is mixed every component: i-TaqTM DNA Polymerase, dNTP mixture, reaction buffer, and so on- in one tube for 1 rxn PCR.

Maxime™ PCR PreMix (i-StarTaq)

Hot start PCR technique was developed as a method to minimize the deleterious effects of mispriming at lower temperatures during PCR. It involves inactivating (or leaving out) one critical component of the PCR reaction until the temperature has risen above this optimal annealing temperature. iNtRON has developed a recombinant Taq DNA polymerase, the i-StarTaqTM DNA Polymerase, which is inactive below an annealing temperature, but can be activated above the annealing temperature.

Maxime PCR PreMix Kit (i-StarTaq) is the product what is mixed every component : i-StarTaqTM DNA Polymerase, dNTP mixture, reaction buffer, and so on- in one tube for 1 rxn PCR.

Maxime™ PCR PreMix (i-pfu)

iPfu DNA Polymerase is a thermostable DNA polymerase purified from an E.coli strain carrying a plasmid with the cloned gene encoding Pyrococcus furiosus DNA polymerase. The enzyme catalyzes the incorporation of nucleotides into duplex DNA in the 5’=>3‘ direction in the presence of Mg2+ at 70-80°C. Pfu DNA Polymerase exhibits 3’=>5‘ exonuclease (proofreading) activity, but has no detectable 5’=>3′ exonuclease activity.

Maxime PCR PreMix Kit (i-pfu) is the product what is mixed every component : i-pfu DNA Polymerase, dNTP mixture, reaction buffer, and so on- in one tube for 1 rxn PCR.

Maxime™ PCR PreMix (i-MAXⅡ)

i-MAXTM II DNA Polymerase enzyme blend combines the proofreading activity of Pfu DNA Polymerase with the high processivity of Taq DNA Polymerase, enabling increased fidelity of PCR amplification and increased yield of PCR amplification.

Maxime PCR PreMix (i-MAXTM II) is the product what is mixed every component: i-MAXTM II DNA Polymerase, dNTP mixture, reaction buffer, and so on- in one tube for 1 rxn PCR.

Maxime™ PCR PreMix (i-StarMAX Ⅱ)

Maxime PCR PreMix Kit (i-StarMAX II) is a product mixed with every component. i-StarMAX II DNA Polymerase, dNTP mixture, reaction buffer, and so on- in one tube.

The i-StarMAX II DNA Polymerase is recommended for relatively rapid, high-fidelity amplification of PCR targets up to 15kb when proofreading DNA polymerase alone requires too long an extension time or yields are insufficient.

Maxime RT PreMix Kits

Maxime™ RT PreMix Kit (Random Primer) is a product that makes cDNA synthesis simple and fast from various RNAs such as prokaryotic and eukaryotic cells. This product is a product that can perform cDNA synthesis process in 1 step in a PCR tube using PCR machine unlike conventional 2 steps cDNA synthesis (RNA denaturation, cDNA synthesis).

Maxime™ RT PreMix (Random Primer)

Maxime™ RT PreMix Kit (Random Primer) contains all components (Random Primer, dNTP mixture, Reaction buffer, etc.) required for RT reaction including OptiScript™ RT System, and it is contained in each PCR tube with dried pellet state by ALHP system. 

Maxime RT PreMix (Oligo dT Primer)

Maxime™ RT PreMix Kit (Oligo dT15 Primer) contains all components (Oligo dT15 Primer, dNTP mixture, Reaction buffer, etc.) required for RT reaction including OptiScript™ RT System, and it is contained in each PCR tube with dried pellet state by ALHP system.

*All products listed on this site and sold through LAB-A-PORTER Limited are for research use only (RUO). Not for human use. Not for therapeutic or diagnostic use.

PRODUCT APPLICATIONS

Use of iNtRON Biotechnology’s products cited in over a hundred of journal articles, covering various research areas including stem cell research, immunology research, oncology research, oral bioscience etc.

FEATURED PRODUCTS

GENOMIC DNA EXTRACTION

 

G-spin™ Total DNA Extraction Mini Kit

G-spin™ Genomic DNA Extraction Mini Kit (Bacteria)

G-DEX™ IIc Genomic DNA Extraction Kit (Cell/Tissue)

i-genomic BYF DNA Extraction Mini Kit

i-genomic Plant DNA Extraction Mini Kit

PLASMIC DNA EXTRACTION & PURIFICATION

 

DNA-spin™ Plasmid DNA Purification Kit

Fast DNA-spin™ Plasmid DNA Purification Kit

DNA-midi™ GT Plasmid DNA Extraction Kit (Gravity)

DNA-maxi™ SV Plasmid DNA Purification Kit

MEGAquick-spin™ plus Total Fragment DNA Purification Kit

RNA EXTRACTION

 

easy-BLUE™ Total RNA Extraction Kit

easy-spin™ Total RNA Extraction Kit

IQeasy™ plus Plant RNA Extraction Mini Kit

RNA-spin™ Total RNA Extraction Kit

Viral Gene-spin™ Viral DNA/RNA Extraction Kit

Patho Gene-spin™ Viral DNA/RNA Extraction Kit

RNase WiPER

PROTEIN EXTRACTION & SYSTEM

 

PRO-PREP™ Protein Extraction Solution (Cell/Tissue)

PRO-PREP™ Protein Extraction Solution (Cell/Tissue) (EDTA free)

PRO-MEASURE™ Protein Measurement Solution

SMART™ BCA Protein Assay Kit

GangNam Stain™ Prestained Protein Ladder (3 Colors)

Miracle-Star™ Western Blot Detection System

NomelRT™ Western Blot Stripping Buffer

PCR PREMIX KITS & DNA POLYMERASES

 

i-Taq™ DNA Polymerase with Buffer, dNTPs

i-StarTaq™ DNA Polymerase with Buffer, dNTPs

i-pfu DNA Polymerase with Buffer, dNTPs

i-MAX™ II DNA Polymerase with Buffer, dNTPs

i-StarMAX™ II DNA Polymerase with Buffer, dNTPs

Maxime™ PCR PreMix Kit (i-Taq)

Maxime™ PCR PreMix Kit (i-StarTaq)

Maxime™ PCR PreMix Kit (i-pfu)

Maxime™ PCR PreMix Kit (i-MAX II)

Maxime™ PCR PreMix Kit (i-StarMAX II)

2X PCR Master mix Solution (i-Taq)

2X PCR Master mix Solution (i-StarTaq)

2X PCR Master mix Solution (i-pfu)

2X PCR Master mix Solution (i-MAX II)

2X PCR Master mix Solution (i-StarMAX II)

RT-PCR PREMIX KITS

 

Maxime™ RT PreMix Kit (Oligo dT Primer)

Maxime™ RT PreMix Kit (Random Primer)

Maxime™ RT-PCR PreMix Kit

ONE-STEP RT-PCR PreMix Kit

HiSenScript™ RH(-) RT PreMix kit

Power cDNA Synthesis Kit

REAL-TIME PCR PREMIX KITS

RealMOD™ Green W² 2x qPCR mix

RealMOD™ Probe W² 2x qRT-PCR mix

RealMOD™ Probe M² 2X qPCR mix

RealMOD™ Probe M² 2X qRT-PCR mix

RealMOD™ Probe M² 2X qPCR mix (with UDG)

RealMOD™ Probe M² 2X qRT-PCR mix (with UDG)

RealMOD™ Probe R² 2X qRT-PCR mix (with UDG)

 

 

CELL CULTURE

iN-fect™ In vitro transfection reagents

NO (Nitric Oxide) Plus Detection kit

e-Myco™ Mycoplasma PCR Detection Kit (ver.2.0)

e-Myco™ plus Mycoplasma PCR Detection Kit

e-Myco™ VALiD Mycoplasma PCR Detection Kit

e-Myco™ VALiD-Q Mycoplasma qPCR Detection Kit

e-Myco™ VALiD-Q2 Mycoplasma qPCR Detection Kit

MycoClean™ Mycoplasma Prevention Spray

*All products listed on this site and sold through LAB-A-PORTER Limited are for research use only (RUO). Not for human use. Not for therapeutic or diagnostic use.

Learn more on our comprehensive offerings on molecular tools.

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