IMMUNOSTEP

As one of the leading providers of technologies, tools, and services for bioscience and biopharmaceutical manufacturing, Immunostep offer a wide range of assays, kits and solutions for Flow Cytometry.

In 2019 Immunostep R&D team recognized the great potential of exosomes and since then they have been working on the development of the best methods for detection, quantification, isolation, characterisation and immunophenotyping of extracellular vesicles (EVs). Featured products include Exosome FACS capture and detection kit, the ExoStep, and exosome isolation products.

Immunostep works in accordance with the standard UNE-EN ISO 13485: 2018 (EN ISO 13485: 2016 & ISO 13485: 2016).

The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound capture antibody and a fluorochrome conjugated detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.

Antibodies are an essential tool for scientists in biomedical and diagnostic research. Antibodies targeted against exosome associated antigens (exosome marker antibodies: CD63, CD9, CD81, etc.) facilitate the characterization and/or quantification of exosomes in cells, tissues or other biological samples.

Immunostep offers a wide range and high quality multi-assay/species validated antibodies for various exosome markers. These antibodies are available in several different conjugated formats, with the right formulation for exosome detection. Besides, these antibodies can be used together with our Exostep Detection kit for exosome subpopulations characterization.

Specific exosomes purification from one cell type or exosome subpopulation characterization remains a challenge. Thanks to our human capture beads, it is possible to isolate specific exosomes among others, from biological fluids (serum, plamas, CSF, saliva, urine, etc.) without previous sample enrichment procedures.

Pure Lyophilized Exosome Standards from human biofluids (plasma, serum) and different cell culture media. Immunopstep lyophilized standards have been validated by WB and FCM, for overall protein content and particle number by Nanoparticles Tracking Analysis (NTA).