Scientific Reports has published an article talking about the use of 3D bioprinting of cancer cell lines to perform drug testing. Below are some highlights from the article:
Conventional evaluation on cancer drug development
- Cancer drug development is usually examined in traditional two-dimensional (2D) cell culture platforms.
- Traditional 2D cultured cancer cells cannot imitate the complexity and heterogeneity of in-vivo tumor.
Hydrogel-based 3D scaffolds
To overcome the cons in traditional 2D cell culture platforms, 3D cell culture platforms are being currently developed.
Hydrogel-based 3D scaffolds are gaining attention because of their cell encapsulation potential and their characteristics of mimicking native extracellular matrix (ECM).
Bioprinting techniques are of interest to tissue engineers because of their ability to construct complex structures.
Use bioink to screen anti-cancer drugs
- In collaboration with TheWell Bioscience, Gebeyehu, and his colleagues tested a series of hydrogels to develop a bioink that mimics the in-vivo tumor microenvironment, shows good printability, rheological characteristics, and biocompatibility.
- They observed that Ink H4 and RGD modified Ink H4-RGD from TheWell Bioscience displayed excellent rheological properties.
- Both bio-inks can be crosslinked with cell culture medium, which aids good cell growth and scaffold stability
- Ink H4-RGD demonstrated printability between 20 and 37 °C
- The scaffolds stayed stable for 15 days at a temperature of 37 °C
- 3D printed non-small-cell lung cancer patient-derived xenograft exhibited speedy spheroid growth of size
- Tumour microenvironment formation within 7 days
- The results of flow property, shape fidelity, scaffold stability, and biocompatibility of H4-RGD recommend that Ink H4-RGD hydrogel could be considered for high throughput screening of various anti-cancer drugs.
1. Gebeyehu, Aragaw, et al. Polysaccharide Hydrogel Based 3D Printed Tumor Models for Chemotherapeutic Drug Screening. Sci Rep, vol. 11, no. 372, 11 Jan. 2021, http://doi.org/10.1038/s41598-020-79325-8.
2. Research Highlights: 3D Printed Tumor Spheroids for Disease Modeling and Chemotherapeutic Drug Screening. TheWell Bioscience, Jun 2021, https://www.thewellbio.com/3d-printed-tumor-spheroids-disease-modeling-chemotherapeutic-drug-screening/