August 19, 2020 |
Have you ever wondered what were the exact indels that were made to the DNA after genome editing experiments? Have you wondered what percentage of the cells had certain edits? Have you ever wondered whether the edits were made on only one chromosome or both chromosomes to reveal the zygosity of the edit? Using single-cell DNA sequencing on the Tapestri Platform, the Wu lab from Dana Farber did just that. Come join us as Dr. Elisa Ten Hacken shares novel data on how they used the Tapestri Platform to better understand their CRISPR mouse models of chronic lymphocytic leukemia (CLL).
A novel platform for single-cell detection of multiple CRISPR-edited gene modifications
Aug 19, 2020 12:30 AM in Hong Kong SAR
Measuring the efficiency of CRISPR genome-editing systems using the Tapestri Platform and Tapestri Single-cell DNA Custom Panels
Genome-editing systems are increasingly used to advance cell therapies, but edited cellular systems need to be thoroughly characterized to fully understand the exact nature of induced mutations. Both on and off-target effects must be measured through high-sensitivity detection methods. Single-cell resolution supports genome-editing system optimization by enabling the detection of low-frequency events – down to 0.1% of cells. Furthermore, single-cell data resolves the complexities of mutation zygosity and co-occurrence in a genome-editing experiment targeting multiple loci. The Tapestri Platform offers a turnkey solution to the complex challenge of characterizing genome-edited systems.