CRISPR has gone from a new tool to one of the most important discoveries in the world of biology. The applications of CRISPR are endless, and there is a plethora of information out there on how this tool can be used in different ways. But Synthego knows that too much choice is often overwhelming! It may be especially hard to figure out which details are pertinent to your specific use case. Secondly, just knowing the potential of CRISPR in several applications does not help gauge whether it is even worth taking up the whole workload of CRISPR or if you need help along the way.

Synthego has generated an interactive tool, Guided Edit, to help you with just that! In this post, we provide a detailed guide for using this tool so that you get the best out of it.

What is Guided Edit

Bearing in mind that information by itself is not necessarily useful unless it is given in bite-sized pieces, Synthego created an interactive tool that allows researchers to answer a series of questions around their CRISPR experiment. The goal is to help them navigate the decision tree, such that they can make an informed decision around the best solution for their specific project.

What information counts as reliable for researchers? Given that academic success largely depends on peer review, Synthego knows that researchers trust the opinions and experiences of one another. Synthego, therefore, surveyed hundreds of CRISPR researchers around time, cost, and efficiency of their CRISPR experiments. Synthego used the results of this survey to inform Guided Edit users at decision forks.

What to Expect From Guided Edit

Before we get into a stepwise tour of the Guided edit tool, here’s an overview of what to expect from this experience. By familiarizing yourself with the sections that will appear on the screen, you can focus on the information that is most important to you while knowing other options, if you need them.

Guided Edit Interactive Tool

Meet Guided Edit, an interactive tool to help you navigate through a clear-cut decision tree, so you arrive at the best solution for your CRISPR editing goal. Start by telling us a bit about your project and answer a few questions.

The Question: Decision Checkpoint

The most important section on the screen that will appear throughout the journey is the question. Synthego has kept the questions simple and specific to what might influence your experimental decisions: edit type, cell type, protein coding targets, etc.

Sidebar: Get More Info

One of the most important aspects of what influences decisions is the consequence. How can one make an informed decision if they are unaware of what the implications of those decisions are. Synthego has therefore kept sidebars for extra info- be it definitions of technical terms or results from our survey to offer users insights into the pros and cons of every choice they make.

Progress Bar: Keep Track of Your way

Synthego has tried to keep the questions to a minimum but we know it can be tiresome- especially if you try out multiple paths. If at any point you feel you have lost track of the journey, we got your back. They have created a progress bar at the bottom, so that you don’t need breadcrumbs to keep track of your way. 

How to Use Guided Edit: Step-by-Step Directions

In this section, we will dive into the details of each step for using this tool. Getting started cannot be simpler- just give your project name (you can name it anything), your email address, and tentative start date and you are good to go. 

Step 1: Pick Your CRISPR Edit Type

It starts with the very basic question of how you plan to use CRISPR in your project. For simplicity, we have only included editing-based CRISPR options: knockout, knock-in, screening, and CRISPRi/CRISPRa. 

Note that in this example, we choose Knockout as an example and demonstrate only one path of the tool. Depending on the choices, some paths may be shorter or longer than others, but the basic elements in all of these are the same.

Step 2: Choose Your Cell Type

The advantage of CRISPR is that it can be used in any cell type, especially with synthetic sgRNA options. Pick your cell type of interest to continue further in the journey. Check out the sidebar for helpful definitions in case you need help in the choice.

Step 3: Tell Us the Target Gene Details

The range of solutions that we offer depends on whether you are planning to knock out a protein-coding gene or not. Protein coding genes consist of a promoter followed by coding information for producing proteins. 

Step 4: DIY CRISPR: Decide if it is Right for You

The next step is to decide whether you want to do the CRISPR experiment yourself or if you prefer quick results by getting Engineered Cells. There is information from our survey around costs and time investment in both options to help you make the decision. Whichever option you choose, we have solutions to help you with your experiment.

Step 5: Pick Your Format of Engineered Cells

If you choose the CRISPR-done-for-you option to get knockout cells, the next question is specifically around the format. You can pick our high-efficiency edited knockout cell pools and isolate clones/use them for assays or pick the premier hands-off-CRISPR option where you get a clonal population of 100% edited cells.

Journey Ends, Destination Begins

Now that you have answered all the questions, the hard part is done. Sit back and let us do the work now. In this section, we will highlight what you will see on the destination page at the end of the Guided Edit journey.

The Best CRISPR Solution for Your Needs

The destination page features the best recommended Synthego product, based on the specific experimental choices that you filled in and dives into details of the specific solution. In this example, it is the Knockout Cell Clones. You can “Download Report” to get these details in your email and even share with us for a quotation.

Advantages of the Chosen Product

As you scroll down on the destination page, you will see a list of advantages of that particular product. These are bullet points summarizing the key features of that product, which makes it easy for you to review what you would get at a quick glance.

Guided Edit Interactive Tool

Meet Guided Edit, an interactive tool to help you navigate through a clear-cut decision tree, so you arrive at the best solution for your CRISPR editing goal. Start by telling us a bit about your project and answer a few questions.


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