Currently, there is a trade-off with CRISPR: technology is very precise, but it is also quite inefficient. Recent studies demonstrated an improvement to this system by introducing small molecules that are able to maintain the precision fo the technology while boosting its efficiency.
PeproTech has expanded its catalog of Small Molecules to include reagents which enhance the overall efficiency and targeting precision of CRISPR/Cas9-mediated genome editing.

Description |
Catalog Number |
Function |
CRISPR-related Actions |
(Z)-4-Hydroxytamoxifen | 6800637 | Estrogen receptor modulator | Activates intein-linked inactive Cas9 to reduce off target effects of CRISPR-mediated gene editing. |
Brefeldin A | 2031560 | Intracellular protein transport inhibitor | Enhances CRISPR-mediated homology-directed repair (HDR) efficiency. |
L-755,507 | 1594312 | Selective β3 adrenergic receptor partial agonist | Enhances CRISPR-mediated homology-directed repair (HDR) efficiency 2-3 fold for large fragments and around 9-fold for point mutations. |
Nocodazole | 3141890 | Microtubule inhibitor | Enhances CRISPR-mediated homology-directed repair (HDR) efficiency. |
NU 7441 (KU 57788) | 5039598 | DNA-PK inhibitor | Enhances CRISPR-mediated homology- directed repair (HDR) efficiency and decreases nonhomologous end-joining (NHEJ) frequency. |
RS-1 | 3127446 | RAD51 activator | Enhances CRISPR-mediated homology-directed repair (HDR) and CRISPR-Cas9 knock-in efficiency. |
SCR7 pyrazine | 1489782 | DNA ligase IV inhibitor | Enhances CRISPR-mediated homology- directed repair (HDR) efficiency and decreases nonhomologous end-joining (NHEJ) frequency. |
Zidovudine (AZT) | 3058716 | Selective reverse transcriptase inhibitor | Decreases CRISPR-mediated homology-directed repair (HDR) efficiency. |
Selected Reference:
Yu, C., Liu, Y., Ma, T., Liu, K., Xu, S., Zhang, Y., … & Qi, L. S. (2015). Small molecules enhance CRISPR genome editing in pluripotent stem cells.Cell stem cell, 16(2), 142-147.
Song, J., Yang, D., Xu, J., Zhu, T., Chen, Y. E., & Zhang, J. (2016). RS-1 enhances CRISPR/Cas9-and TALEN-mediated knock-in efficiency. Nature communications, 7.