Improve your CRISPR Gene Editing with Small Molecules

by | Nov 12, 2019 | CRISRP, Stem Cell

Currently, there is a trade-off with CRISPR: technology is very precise, but it is also quite inefficient. Recent studies demonstrated an improvement to this system by introducing small molecules that are able to maintain the precision fo the technology while boosting its efficiency.

PeproTech has expanded its catalog of Small Molecules to include reagents which enhance the overall efficiency and targeting precision of CRISPR/Cas9-mediated genome editing.

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Description

Catalog Number

Function

CRISPR-related Actions
(Z)-4-Hydroxytamoxifen 6800637 Estrogen receptor modulator Activates intein-linked inactive Cas9 to reduce off target effects of CRISPR-mediated gene editing.
Brefeldin A 2031560 Intracellular protein transport inhibitor Enhances CRISPR-mediated homology-directed repair (HDR) efficiency.
L-755,507 1594312 Selective β3 adrenergic receptor partial agonist Enhances CRISPR-mediated homology-directed repair (HDR) efficiency 2-3 fold for large fragments and around 9-fold for point mutations.
Nocodazole 3141890 Microtubule inhibitor Enhances CRISPR-mediated homology-directed repair (HDR) efficiency.
NU 7441 (KU 57788) 5039598 DNA-PK inhibitor Enhances CRISPR-mediated homology- directed repair (HDR) efficiency and decreases nonhomologous end-joining (NHEJ) frequency.
RS-1 3127446 RAD51 activator Enhances CRISPR-mediated homology-directed repair (HDR) and CRISPR-Cas9 knock-in efficiency.
SCR7 pyrazine 1489782 DNA ligase IV inhibitor Enhances CRISPR-mediated homology- directed repair (HDR) efficiency and decreases nonhomologous end-joining (NHEJ) frequency.
Zidovudine (AZT) 3058716 Selective reverse transcriptase inhibitor Decreases CRISPR-mediated homology-directed repair (HDR) efficiency.
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Selected Reference:

Yu, C., Liu, Y., Ma, T., Liu, K., Xu, S., Zhang, Y., … & Qi, L. S. (2015). Small molecules enhance CRISPR genome editing in pluripotent stem cells.Cell stem cell, 16(2), 142-147.

Song, J., Yang, D., Xu, J., Zhu, T., Chen, Y. E., & Zhang, J. (2016). RS-1 enhances CRISPR/Cas9-and TALEN-mediated knock-in efficiency. Nature communications, 7.

Davis, K. M., Pattanayak, V., Thompson, D. B., Zuris, J. A., & Liu, D. R. (2015). Small molecule-triggered Cas9 protein with improved genome- editing specificity. Nature chemical biology, 11(5), 316-318.

Robert, F., Barbeau, M., Éhier, S., Dostie, J., & Pelletier, J. (2015). Pharmacological inhibition of DNA-PK stimulates Cas9-mediated genome editing. Genome medicine, 7(1), 93.