Annexin V is a phospholipid phosphatidylserine (PS) binding protein. In the earlier stages of apoptosis, PS is translocated to the outer sections of the cell plasma membrane and is exposed to the external cellular environment. The mark of the late stages of cellular death is the loss of membrane integrity. Cells can be stained with Annexin V conjugated to a fluorochrome together with 7-AAD, which is excluded from viable cells with intact membranes. This can be used to distinguish cells that are viable (Annexin V and 7-AAD negative), early apoptotic (Annexin V positive and 7-AAD negative), and those that are dead or in late apoptosis (Annexin V and 7-AAD positive).
The Annexin V Apoptosis Detection Kit contains Annexin V PE/FITC/APC (100 tests), Annexin V Binding Buffer 1X (50mL), and 7-AAD (200 tests).
Aqueous buffered solution containing 1mM EDTA, 10mM EPES, 0.09% NaN3.