Now is the time to shift from Matrigel™ to Animal Free Vitronectin cell culture surface-coating reagent

The extracellular matrix (ECM) plays an important role in the stem cell niche. In-vitro stem cell cultures have utilized feeder cells and an undefined, animal-derived ECM. Current stem cell research needs calls for defined culture conditions, including the use of defined recombinant ECM.

Kit Components (Cat# AF-VMB-220):
1. Animal-Free Human Vitronectin Matrix
2. Animal-Free Vitronectin Matrix Buffer (PBS + Kolliphor P 188) for Vitronectin Matrix reconstitution and cell plate/dish dilutions. Kolliphor P 188 is a solubilizer that acts as a dispersing and wetting agent for optimal coating of cell culture vessels with vitronectin.

The kit allows the coating of 100 wells of 6-well plates with vitronectin.

The reference medium produces well-formed tightly packed colonies, on both Matrigel and Vitronectin. Cells grown in the PeproGrow-hESC medium on both matrices retain the same morphological characteristics of the cells grown in the reference medium.ngs.

DAPI: Nuclear counterstain
Nanog: Pluripotency marker, a transcription factor expressed in undifferentiated cells
Oct4: Pluripotency marker, a transcription factor expressed in undifferentiated cells
TRA-1-60: Pluripotency marker, expressed on the surface undifferentiated cells

DAPI: Nuclear counterstain
Oct4: Pluripotency marker, a transcription factor expressed in undifferentiated cells
SSEA1: Differentiation marker, expressed on the surface differentiated cells
SSEA4: Pluripotency marker, expressed on the surface undifferentiated cells